EHP Infected Pond Preparation: Earthen Ponds

01 — Overview

What this protocol is for

This protocol prepares earthen ponds with a confirmed EHP (Enterocytozoon hepatopenaei) infection history for restocking. It uses a high-pH lime treatment followed by bioremediation to eliminate EHP spores. The acre version uses MinGroX in the final step. The hectare version uses MinGro.

02 — Indications

When to use this protocol

Earthen ponds with EHP history, White Shrimp or Tiger Shrimp.

03 — Procedure

Step-by-step instructions

1. 1

   Till soil and apply lime

   Till the soil 10 cm deep. Apply 2 to 4 tonnes of burnt lime (CaO) per acre (or 5 to 10 tonnes per hectare). Fill to 50 cm. Target pH at or above 8.5, preferably 9. Leave for 5 to 7 days. Discharge water.

2. 2

   First bioremediation step

   Fill to 30 cm. Apply SoilGro 800 g per acre (or 2 kg per hectare) with jaggery 8 kg per acre (or 20 kg per hectare). Aerate. Leave for 4 to 5 days.

3. 3

   Second bioremediation step

   Top up by 30 cm. Apply SoilGro 800 g per acre (or 2 kg per hectare) with jaggery 8 kg per acre (or 20 kg per hectare). Aerate. Leave for 4 to 5 days. Discharge water.

4. 4

   Fill and second lime application

   Fill to culture depth. Apply burnt lime at 800 kg per acre (or 2 tonnes per hectare). Target pH 8.3 to 8.4 at stocking.

5. 5

   Disinfection

   Apply AlphaSep at 3 ppm. Leave for 2 days.

6. 6

   Mineral correction and pre-stocking check

   If salinity is below 15 ppt, apply MinGroX at 8 kg per acre for 3 consecutive days. If using the hectare version, apply MinGro at 17 kg per hectare instead. Confirm Secchi disk reading is at or above 50 cm before introducing PLs.

04 — Parameters

Key parameters to monitor

05 — Products

Products used in this protocol

Each product name links to its Blue Aqua product page for specifications and purchasing.